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Apply for Job Job ID361904 LocationTwin Cities Job FamilyResearch-Researchers Full/Part TimeFull-Time Regular/TemporaryRegular Job Code8352R1 Employee ClassCivil Service Add to Favorite Jobs Email this Job About the Job Job Duties: Incumbent's responsibilities will involve the management and evaluation of experimental cellular therapies for diabetes using mouse models; this will include genotyping (PCR), drug preparation and delivery and dissection of mice upon trial completion; play a role in trial evaluation via protein and RNA analysis including histology, immunohistochemistry, cell culture, cellular assays, RT-PCR, Western blot analysis, and intraviteal cellular imaging. Scientist will help use the data collected from these evaluation methods to initiate subsequent trials; assist in normal lab and mouse colony maintenance and organization; and perform other duties as assigned. Maintain unique transgenic and knockout stocks and strains of animals; responsible for maintaining breeding operations and records and interacting and collaborating with research staff; will participate in monitoring rodent populations by PCR, Flow Cytometry, and diabetes assessment prior to and following treatments. (50%) Perform research projects according to established protocols and following direction of PI to include: Mab production, cell culture, drug preparation, tolerance experiments, diabetes assessment, plasmid transfection, drug preparation and administration, assist with PBMC and tissue preparations, assist with surgical procedures, and perform flow cytometry analysis, immunohistochemistry, and cellular imaging 2-photon microscopy (10%) Lab organization, supply ordering, lab cleaning, records keeping, solutions and equipment maintenance (30%) Data collection and analysis. Prepare written and graphical descriptions of results to discuss with PI. Review scientific literature and technical documents. (10%) Qualifications Required Qualifications: (Must be documented on all application materials) B.S. or B.A. in biological sciences, related field, or education and work experience in biological science to equal a combination of 4 years. Experience working with mice including handling mice in specific pathogen free (SPF) conditions and tissue sample collection. Ability to harvest mouse tissues and sterile cell culture techniques. Mouse monitoring skills required include general animal health under normal conditions and following diabetes induction are also required. Immunology research experience or course work with a passing grade is required. Substitute topics could be considered. Multicolor flow cytometry skills and experience. The applicant must be able to interact professionally with all faculty, students and staff who utilize the facility. Computer skills include word-processing, spreadsheets, database and presentation, preferably with Microsoft Office Software. Excellent organizational skills, communication, and attention to detail is also required. Preferred Qualifications: Animal husbandry skills, data entry and database management are also preferred. Experience with injections via IV, IP, and SQ immunizations and daily monitoring of diabetes in NOD mice using urine and blood glucose analysis and administration of insulin or saline. Experience and skills include biological/cellular imaging, mononuclear cell enrichment from PBMC and human tissues, PCR troubleshooting, Western blot analysis, aseptic technique and assistance with surgical procedures. Ordering lab reagents, daily lab maintenance, and preparing solutions is highly desirable. Flow cytometry skills and analysis techniques are highly desirable. Benefits Working at the University At the University of Minnesota, you'll find a flexible work environment and supportive colleagues who are interested in lifelong learning. We prioritize work-life balance, allowing you to invest in the future of your career and in your life outside of work. The University also offers a comprehensive b nefits package that includes: Competitive wages, paid holidays, and generous time off Continuous learning opportunities through professional training and degree-seeking programs supported by the Regents Tuition Benefit Program Low-cost medical, dental, and pharmacy plans Healthcare and dependent care flexible spending accounts University HSA contributions Disability and employer-paid life insurance Employee wellbeing program Excellent retirement plans with employer contribution Public Service Loan Forgiveness (PSLF) opportunity Financial counseling services Employee Assistance Program with eight sessions of counseling at no cost Please visit the Office of Human Resources website for more information regarding benefits. How To Apply Applications must be submitted online. To be considered for this position, please click the Apply button and follow the instructions. You will be given the opportunity to complete an online application for the position and attach a cover letter and resume. Additional documents may be attached after application by accessing your "My Job Applications" page and uploading documents in the "My Cover Letters and Attachments" section. To request an accommodation during the application process, please e-mail employ@umn.edu or call (612) 624-8647. Diversity Employment Requirements Any offer of employment is contingent upon the successful completion of a background check. Our presumption is that prospective employees are eligible to work here. Criminal convictions do not automatically disqualify finalists from employment. About the U of M The University of Minnesota, Twin Cities (UMTC) The University of Minnesota, Twin Cities (UMTC), is among the largest public research universities in the country, offering undergraduate, graduate, and professional students a multitude of opportunities for study and research. Located at the heart of one of the nation's most vibrant, diverse metropolitan communities, students on the campuses in Minneapolis and St. Paul benefit from extensive partnerships with world-renowned health centers, international corporations, government agencies, and arts, nonprofit, and public service organizations. At the University of Minnesota, we are proud to be recognized by the Star Tribune as a Top Workplace for 2021, as well as by Forbes as Best Employers for Women and one of America's Best Employers (2015, 2018, 2019, 2023), Best Employer for Diversity (2019, 2020), Best Employer for New Grads (2018, 2019), and Best Employer by State (2019, 2022). The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds. The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: http://diversity.umn.edu.